Unaltered strong immunohistochemical expression of CD44-v6 and -v5 isoforms during development and progression of oral squamous cell carcinomas

The present study was designed to immunolocalize CD44-v6 and -v5 isoforms in normal, dysplastic and malignant oral mucosa as well as in primary and meta-static oral squamous cell carcinomas. Routinely formalin-fixed and paraffin-embedded tissues of 100 oral carcinoma patients were immunohistochemically investigated following wet autoclave antigen retrieval. Both CD44-v6 and -v5 epitopes were uniformly strongly expressed on the cell surface of basal and intermedial epithelial cells of normal and dysplastic mucosa and in all primary and melastatic squamous cell carcinomas with the exception of end-differentiated keratinizing cells. Our results strongly suggest that CD44-v6 and-v5 isoform expression is not altered during development and progression of oral carcinomas. Thus, they seem to be irrelevant factors in the prediction of prognosis in this type of cancer.     

Do buffered local anesthetics provide more successful anesthesia than nonbuffered solutions in patients with pulpally involved teeth requiring dental therapy?: A systematic review

Background

The authors conducted a systematic review that addresses the following population, intervention, comparison, outcome question: “In adults requiring dental therapy with pulpally involved teeth, what is the comparative efficacy of buffered local anesthetics (LAs) compared with that of nonbuffered LAs in achieving anesthetic success?”

电子病历智能检索系统设计与实现

以海南省人民医院为例,阐述电子病历智能检索系统设计思路、技术路线、架构以及具体功能,指出该系统应用有助于提升对临床科研的支撑保障能力,为实现精准医疗和智慧医疗服务体系奠定坚实基础。     

A comparison of two microcomputer-based programs for bibliographic retrieval and formatting

We evaluated two representative microcomputer-based programs for organizing a biomedical literature filing system. With a bibliography of 100 anesthetic references, a series of benchmark tests was developed to measure the speed and accuracy of typical searching, sorting, and formatting tasks. Each program performed the searching tasks accurately and at about the same speed. One program performed sorting without errors, provided the field order of the template used to enter references was unchanged. Both programs used punctuation files, that is, templates for controlling author presentation; punctuation to suit style requirements of individual journals; and order of particular fields, such as publisher and year of publication. Each program was able to format journal, book, and chapter references correctly, but the resulting output required some refining in a word processor. Both require a major time commitment to learn and to create custom punctuation files for journals not included in the predesigned punctuation files. Once mastered, both programs are quite competent at organizing reprints and formatting journal references.     

护理人员信息行为研究

正确有效的信息行为是开展循证护理和护理研究的重要保障。根据Wilson信息寻求行为模型,将护理人员信息行为分为信息需求、信息查询、信息筛选、信息利用4个组成部分。并从情报学角度探讨信息行为各部分的具体表现及影响因素,从加强护理人员素质培养和信息建设两方面提出保障有效信息行为的措施。     

Component Size Mismatch of Metal on Metal Hip Arthroplasty: An Avoidable Never Event

Size mismatch of components used in total hip arthroplasty is a serious, preventable patient safety incident of unknown prevalence as many cases are not detected. Component size mismatch was found in 11 cases (0.9%) at our retrieval centre. All cases of mismatch were not detected on plain radiograph during routine clinical follow up and blood metal ion levels were elevated above the MHRA action level of 7 ppb. Root cause analysis identified manufacturer, hospital and surgeon factors that need to be addressed to reduce the incidence of this avoidable clinical problem. Retrieval analysis is the only method of confirming size mismatch and is likely to be under-represented in National Joint Registries that record the indication for revision at the time of revision.     

Zirconia Phase Transformation,Metal Transfer,and Surface Roughness in Retrieved Ceramic Composite Femoral Heads in Total Hip Arthroplasty

Ceramic femoral heads have had promising results as a bearing surface in total hip arthroplasty. Our objective was to evaluate a series of retrieved alumina-zirconia composite ceramic femoral heads for evidence of the tetragonal to monoclinic zirconia phase transformation, metal transfer and articular surface roughness. Raman spectra showed evidence of the zirconia phase transformation in all retrieved specimens, with distinct monoclinic peaks at 183, 335, 383, and 479 cm^− 1. All components displayed metal transfer. An increase in the zirconia phase transformation was seen with increasing time in vivo. No correlation between extent of zirconia phase transformation and the surface roughness was found. These short-term results suggest that the use of an alumina-zirconia composite ceramic is a viable option for femoral heads in THA.     

几种抗原修复方法研究

由于常规福尔马林固定和石蜡包理过程造成的抗原封闭与结构改变，限制了一些抗体在免疫组织化学中的应用。为了恢复组织的抗原性，提高免疫组织化学的检测阳性率，我们应用高压锅，微波炉和胰蛋白酶分别对石蜡切片进行处理。结果显示，一些抗体过去只能用于冰冻切片，经前处理后可用于石蜡切片，一些抗体的敏感性增强，经高压锅处理的免疫染色重复性较微波炉处理得好，个别抗体适用于酶消化。总之，在免疫组织化学染色过程中应根据抗原抗体的特性选用适当的前处理方法。